Biochemical genetic polymorphisms were investigated in the Egyptian and Carniolan races and their Hybrid genotype. Esterase isozyme bands showed that, whereas Est-C: band appears in common among all populations studied and cannot be considered as a genotypic marker, Est-A1 band was restricted to the Egyptian race. However, Est-A2 band appeared in common in both the Carniolan and the Hybrid indicating a closer genetic relationship between them. The distribution of Malate dehydrogenase electrophoretic pattern indicated that two third of Mdh isozymes were common in the three populations studied, while the remaining third was common in both the Carniolan and Hybrid indicating a closer genetic relationship between them. Restricted bands were represented by the extremely high molecular weight bands denoting the Egyptian race (Bands 1 and 2) and the 53 54 Kda band which strictly appeared only in Carniolan and Hybrid slots. These results interprets the presence of obvious genetic variations between Egyptian and Carniolan races and that'the Hybrid is closely related to the Carniolan rather than to the Egyptian race. Comparisons between protein fractions ofthe Hybrid with both in Egyptian and Carniolan races proved that the great majority of the Hybrid fractions have been transmitted from the Carniolan race. However the remaining Hybrid fractions were transmitted from the Egyptian race. These results interpreted the higher similarity between Hybrid and the Carniolan race and may indicate that this Hybrid had been obtained from Carniolan queens mated by Egyptian drones.