Response of M. javanica infected soybean plants to seed soaking in aqueous solutions of ascorbic acid, indole acetic acid, lysine and thiamine, at 500 and 1000 ppm for five hours before sowing was studied in a pot experiment under greenhouse conditions at 27±5°C. Results indicated that the tested chemicals obviously suppressed galling and reproduction of M. javanica, since number of galls and egg-masses on roots and infective juveniles in soil as well as rate of nematode reproduction were significantly decreased in soaked seed treatments. Seed soaked in 1000 ppm gave relatively higher effect compared to 500 ppm. With respect to the effect on certain plant growth parameters, results clearly indicated that all treatments alleviated the inhibitory effect of M. javanica and inhanced growth of soybean plants.                 Regarding to chemical analysis , it was found that as the concentration of the tested compounds increased from 500 to 1000 ppm, free phenols were increased, except in thiamine treatment, while amount of total phenols did not show certain trend. On the other hand, increasing concentration obviously increased reducing sugars, except in indole acetic acid treatment which showed slight increase in reducing sugars. However, opposite trend was noticed with total amino acids, since increasing concentration decreased total amino acids in all treatments.