Necrotic spots on pepper fruits consider a typical symptom of bacterial spots disease.Xanthomonas euvesicatoria isthe causative agent for this bacterial disease on pepper. Colonies isolated from symptomatic pepper fruits were exhibited typical growth on nutrient agar(NA), yeast dextrose calcium carbonate(YDC) and mTMB media. Colonies were convex, mucoid, glistening, wet and circular with an entire edge with yellow pigment.The bacterial isolates were matched with Xanthomonas bacteria in microscopical and biochemical tests. The isolates showed weakness in the hydrolysis of starch and negative in degrade pectate. Leaves of pepper plants exhibited water-soaked as a first reaction in the pathogenicity test. The spots turned to dark necrotic lesions and may fall and the leaves became perforated. On the stem, necrotic lesions were appeared then turned to dark color also the spots on surface fruits turned to dark color. Confirmation of the identity of the pathogenic isolate was performed by BSXeF/BSXeR as specific primers for X. euvesicatoria whichwas showed amplification of a 173bp fragment. BLAST in the NCBI GenBank database with the sequence of 16S rDNA indicated that the nucleotide sequence of a bacterial isolate obtained from the bacterial spot of pepper fruits in Egypt was 96.49% homologs to X. euvesicatoria. The nucleotide sequence of the tested isolate was deposited in NCBI GenBank with accession number MZ540733. X. euvesicatoria survived in pepper plant debris for one month to nine months while cannot survive in seed for the same time. X. euvesicatoria induced the symptoms on tomato, ground cherry, and London rocket plants.