Three pollen types collected by honeybee were chosen (German ,Chinese and  Egyptian pollen ) to study their effects on royal jelly production of honeybee colonies (F1 Carniolan hybrid) and study the chemical composition of pollen types on royal jelly produced. From the results the following aspects could be concluded:  1- Feeding with 200 g/ week German pollen gave the largest mean quantity of royal jelly g/ colony during the study period. 2-No significant appearance was found between the  feeding with 100g / week and 200 g/ week German pollen. 3- Feeding with Chinese pollen gave the same results of German pollen. Total lipid values of German, Chinese and Egyptian pollen were 12.0, 10.3 and 9.2 g/100g pollen, nitrogen content values of pollen were 5.0, 50 and 5.7 g /100g., crude protein were 31.25, 31.25 and 35.6 g/100g pollen ,respectively. Also the carbohydrates values were 48.0, 46.7 and 47.2 g/100g pollen ,respectively. The total lipid values of royal jelly were 8.3, 8.59 and 8.54 g/100g royal jelly, nitrogen content values of royal jelly were 1.6, 1.5 and 1.5g/100g, crude protein of royal jelly were 10.0,9.3 and 9.3 g/100g royal jelly, the carbohydrates values of royal jelly were 10.1, 9.3 and 11.5 g/100g royal jelly ,respectively , the pH of royal jelly were 3.81, 3.90 and 3.92 ,respectively. While, the moisture values of royal jelly production from German ,Chinese and Egyptian pollen fed honeybee colonies were 69.4, 66.5 and 68.5 % royal jelly ,respectively .