In this work the effect of the exposure time of the sealed queen cells to the worker bees was investigated. For this purpose two Carniolan bee colonies were chosen, one being the mother of the queen larvae and the other as rearing colony. After they had been sealed, the queen cells were divided randomly into five groups according to the period they have been staying in the rearing colony (48, 72, 96, 120 and 144 hour), then they were transferred to an incubator at 34 C until their emerge. For each group the rate of emerge was calculated, then the virgin queens were weighted, and dissected for measuring the length and width of the right fore wing and for counting the ovarioles of the right ovary. The results indicated that only the number of ovarioles was correlated positively with the different exposure periods times to the worker bees in rearing colony.