Bee venom was collected from different races and hybrids bees using high quality of the electric shock device. The bee venom was collected every 4&7 days interval during different seasons of the year. Egyptian race and Craniolian hybrid recorded highest venom values among other tested bee races during spring, summer and winter seasons particularly at 4 days interval collection periods. Increases in some biological activities of honeybee colonies resulted collected bee venom were recorded with same collection period. Collected bee venom in the winter season was preferred every 7 days intervals.  There weren't clearly effects resulted both two venom collection periods or race of bees on some bee venom analysis, while total bee venom proteins were related with the season activity.