The present work was carried out at branch Beekeeping Research Department at Qalubia governorate. The aim of investigation evaluate the efficacy of some local natural substances for controlling Varroa destructor in honeybee colonies and their comparison with Apiguard 25% Thymol as material import was recommended. The result obtained show that, the mean reduction of infection with Varroa mite could be arranged into the following descending orders as follows: 86.78%, 81.48%, 79.33%, 75.33% and 68.96% when using plates Apiguard, Crystal Thymol, mixture of (Thymol, Menthol and Camphor), and Menthol, respectively. The experimental colonies were treated with ( 2-plates /colony) one plate at fortnight for (4 weeks). The mean increase of treated honeybee colonies for controlling Varroa mite was 7.96% and 67.69% for both brood rearing and honey production as compared to that of untreated colonies. Generally, it could be concluded that, in cas of infestation of honeybee colonies with Varroa mite it is advisable to use Apiguard substance, Crystal Thymol and a mixture of (Thymol, Menthol and camphor). At the rate of one plate/ 2weeks/ colony for (4weeks) at high temperature greater than 27ºC.