The fertilization have an important role in the abundance of different mite species  and spider species associated with different crops. The results revealed the presence of 28 different mite species belonging to 14 families related to four suborders, Astigmata, Prostigmata, Mesostigmata and Cryptostigmata, associated with soybean leaves. It was found that the abundant families were Tetranychidae, Phytoseiidae, Tydeidae and Tarsonemidae with great number on leaves of soybean treated with chemical fertilization than those treated with  biofertilizer once allover the study periods. Also, the study proved that the total numbers of soil mites in chemical treated plots were more than of plots treated with biofertilizer once, with the exception of mesostigmatid mites were higher in biofertilizer soils. Also data shows that the presence of 16 spider species belonging to 12 families collected by both plant shaking and pit-fall traps methods. The dominant collected families on plants were Araneidae, Dictynidae, Linyphidae, Tetragnathidae and Therididae. The highest density of spiders were observed during September pre- harvest time of crop, and spiders density in chemical fertilization location were higher than those of biofertilization treatment in shaking plant, while the commonest spiders families in soil were Gnaphosidae, Lycoseidae and Oecobiidae, and the population on chemical fertilizer plots were higher than those of biofertilizer once in pit-fall traps. The highest population was noticed for Gnaphosidae.