The present work was carried to study some morphometrical and biological characters affecting of high quality queens. Highest percentages of acceptance, emerged queens, weight of virgin queens, morphometrics of virgin queens and number of ovarioles as well as highest quality of virgin queen rearing were reared and incubated in colonies fed on dried brewer's yeast cake beside stored pollen in combs and sugar syrup 66.6% Conc.. Double grafting technique was the highest percentages of acceptance and emergence of queens, followed by wet grafting technique and dry grafting method came the latter for the previous parameters. The percentages of accepted larvae of virgin queens in the experimental colonies was affected by the type of cell cups tested. Superior of most morphometrics and biologic characters of virgin queens when bee wax cell cups were used than the other cell cups were tested such as plastic cell cups and cell cups made of wax foundation. The position of bar in holder queen rearing affected of some parameters in produced virgin queen rearing were achieved. Highest percentages of acceptance, emergence queens, weight of virgin queen and number of ovarioles were found in virgin queen reared and situated in center of queen rearing holder, followed by those reared in the top holder and the inferior were those reared in the bottom holder of queen rearing. Highest quality of virgin queens were reared and incubated in colonies contained worker population densities over 9 comps covered with bees on each other sides.