The present study was carried out during 2007, 2008 in the apiary of faculty of Agriculture, Al-AzharUniversity at NaserCity. This study compared the nitrogen content of worker bee body produced by different races and their hybrids. From data obtained during spring and summer, it was found no significant different among nitrogen level of worker bee body resulted from different races and their hybrids during spring and summer, while it was significant difference in late summer. The nitrogen content of worker bee body produced by different races and their hybrids were 11.85% and 10.09% for Italian and Carniolan race, while it was 11.10% and 9.74% for both Italian F₁ and Carniolan F₁ respectively. From above data it is clear that the biological and economic value of the locally of Italian race in both summer and late summer season in more obvious and statistically significant. Therefore they may recommended for use in the commercial a piaries.. It is also true that the biochemical variation can be useful in separating at least some population within Apis mellifera, L.