Isolation and identification of the causative pathogen bacteria of the AFB (Paenibaccillus larvae) from the Egyptian honey bee colonies were conducted in this study. The identification was achieved by many tests which assured the pathogen causative. This tests were Roby tests, Holst milk test, selective medium, catalase production, hydrolysis of starch and microscopic examination which involved negative stain, spores staining and Gram stain test. Also a comparison between Egyptian, Bulgarian propolis ethanolic extract and three antibiotics which were tylosin, penicillin and combination of ampicillin and gentamicin from the standpoint of their bacteriostatic effect on three bacterial species which were Paenibaccillus larvae, Listeria monocytogenes and Staphylococcus aureus the comparison was conducted by the technique of measuring the inhibition zone diameter (IZD). The obtained results proved that the Bulgarian propolis ethanolic extract was more effective than local propolis ethanolic extract. Also the S. aureus was more sensitive to propolis ethanolic extract (PEE) than L. monocytogenes, while P. larvae was more resistant for PEE.