This study was carried out to evaluate the efficacy of two bio-control agents, mycorrhizal fungi and the endoparasitic bacterium Pasteuria penetrans in managing development of root-knot nematode, Meloidogyne javanica on tomato. Use of both bio-agents P. penetrans and mycorrhizal fungi, either alone or combined, reduced significantly all related nematode parameters on tomato plants infected with M. javanica. The reduction in galls, egg masses, females/root system and eggs/egg mass as well as juveniles in soil was highly significant when both bio-agents inoculated together as reached 76; 68; 74; 47 and 73%, respectively. Consequently, nematode infected plants that were treated with both bio-agents showed improvement in vegetative plant growth characters as reflected by a significant increase in the fresh shoots and roots as well as dry shoot weights of tomato compared with untreated plants. Moreover the contents of Nitrogen, Phosphorus and Potassium in tomato plants treated with both bio-agents were also increased compared with those found in untreated nematode infected plants. The overall disease symptoms caused by nematode on tomato was markedly alleviated in the pots treated with the tested bio-agents.