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217498

CONTROL OF SEED-BORNE BEAN PATHOGENS WITH SOME ANTAGONISTIC FUNGI AND NON FUNGICIDAL TREATMENTS.

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Last updated: 22 Jan 2023

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Abstract

Ten associated fungi of bean seeds were isolated, purified and identified as Alternaria alternata, Aspergillus spp < em>., Botrytis cinerea, Fusarium solani, Macrophomina phaseolina, Penicillium sp < em>., Rhizoctonia solani, Sclerotinia sclerotiorum, Trichoderma viride and Verticillium spp < em>. Four tested fungal isolates (R. solani, M. phaseolina, F. solani, and S. sclerotiorum) were proved to be pathogenic producing different degrees of pre-emergence and post-emergence damping-off and root rot symptoms on bean cultivars, namely; Contender, Narina, Bolista and Giza-6. The most virulent isolates inducing PRD were F. solani, M. phaseolina and R. solani whereas S. sclerotiorum was the least virulent. Moreover, the highest levels of PTD were obtained with S. sclerotiorum, F. solani, and R. solani, whereas M. phaseolina was the least virulent. Bolista cultivar was the most compatible with PRD and PTD in all tested pathogenic fungi, whereas Giza-6 showed the lowest compatibility. The highest percentage of root rot infection were obtained in Bolista cultivar in tested fungi ranged from (70-80%) and Narina (70-75%), while the least infection was obtained in Giza-6 (55-60%). Plant oils (Cinnamon, Clove, Spearmint and Lemon) were in vitro tested for their antifungal activity against the four tested pathogenic fungi. They significantly reduced the radial growth was obtaining. Spearmint oil exhibited the highest antagonistic effect to the tested fungi followed by Clove and Cinnamon oils, while the least effect was Lemon oil as compared with control. Hyphal growth of R. solani and S. sclerotiorum was completely inhibited by 100% and 75% conc. of Spearmint oil, while R. solani was completely inhibited by 100% conc. of Cinnamon oil. Also, the biological agents Trichoderma viride, T. harzianum, T. koningii and Bacillus subtilis were used in vitro to evaluate their effectiveness against the four tested pathogenic fungi. Linear growth experiments showed that all biological agents were significantly reduced the linear growth of fungi. T. harzianum showed highly antagonistic effect, the growth reduction ranged from (75.6 to 77.8%), while B. subtilis was the least, growth reduction ranged from (56.7 to 65.6%) to the four tested pathogenic fungi.

DOI

10.21608/jppp.2009.217498

Keywords

antagonistic fungi, Biological agents, Bean seeds, Damping-off pathogens, plant oils, Bacillus subtilis, Root-rot pathogens, Trichodermat spp

Authors

First Name

G.

Last Name

Ibrahim,

MiddleName

H.

Affiliation

Plant Pathology Research Institute, ARC, Giza, Egypt

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Orcid

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First Name

Laila

Last Name

Abd-El-Razek

MiddleName

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Affiliation

Plant Pathology Research Institute, ARC, Giza, Egypt

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Orcid

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First Name

Nadia

Last Name

El-Safawani

MiddleName

A.

Affiliation

Plant Pathology Research Institute, ARC, Giza, Egypt

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Volume

34

Article Issue

11

Related Issue

30942

Issue Date

2009-11-01

Receive Date

2009-10-05

Publish Date

2009-11-01

Page Start

10,687

Page End

10,698

Print ISSN

2090-3677

Online ISSN

2090-3758

Link

https://jppp.journals.ekb.eg/article_217498.html

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https://jppp.journals.ekb.eg/service?article_code=217498

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Original Article

Type Code

888

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Journal

Publication Title

Journal of Plant Protection and Pathology

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https://jppp.journals.ekb.eg/

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Article

Created At

22 Jan 2023