The present studies were carried out at Etay El-Baroud Agricultural Research Station during 2006/2007 and 2007/2008 of growing seasons in addition to a pot experiment under controlled conditions during 2007/2008. M14 and M15 generations of six mutant lines derived from three radiated varieties ( Giza-1, Giza-2 and Dijon-2 ) in addition to varieties Belpies-9 and Sohag 2 were evaluated against pre-, post- emergence damping-off, root rot and wilt diseases. Under artificial infection with Fusarium oxysporum f. sp < em>.  lupini, mutant lines number 23, 33 and 37/3 in addition to Giza- 1 and Dijon- 2 had the lowest pre- emergence damping- off % with averages of 0.00% for the mutant 23 and 6.95% for the other four genotypes. In case of the post- emergence damping- off, the mutant line number 37/3 and varieties Giza-1, Dijon- 2 and Behpis-9 were not affected (0.00%). The mutant lines 23, 37/3 followed by Giza-1 and Dijon-2 had the highest survival plants percentages. The disease index of wilted plants ranged between 1.26 and 2.07 and showed no significant differences between the all tested genotype. The mutant lines number 23, 33, 35/4 and 37/3 showed the highest values of shoot length, plant fresh weight, plant dry weight and seed yield/pot compared to their origin varieties. Under filed conditions, the best values of the pre-, post- emergence damping- off, root rotted and wilted plants% and survival plants% were cleared with the mutant lines number 23 and 37/3 in addition to Dijon-2 and Sohag-2 in both seasons. Also, the mutant lines number 37/3 and 23 in addition to Sohag-2 had the best values of number of pods /plant, weight of 100 seeds and seed yield/plot in the two growing seasons.