Fusarium wilt disease is among the serious diseases that attack different tomato cultivars causing loss of stand yield. Pathological and Molecular variations among certain isolates of Fusarium oxysporum lycopersici, collected from diseased tomato plants grown in different governorates of Egypt, were studied in this investigation. The pathogenicity tests were performed for the isolates F. oxysporum f.sp. lycopersici which isolated from samples of tomato roots cv. Castle Rock.  These isolates were collected from different locations in Egypt (El-Gharbiya, El-Qalubia, Beni-Sueif, El-Nubaria, South Tahrir, Kafr El-Sheikh, El-Salhiya, Giza, Al-Arish, El-Ismailia, Sohag and El-Fayoum). The results showed that all isolates were pathogenic, with different degrees of pathogenicity. The electrophoretic study of F. oxysporum f.sp < em>. lycopersici had high degrees of similarity and no relation between the protein profiles and the geographic source. On the other hand, the fingerprinting technique with DNA through using RAPD-PCR showed that there was no clear relationship between genetic and geographic origin.