The research is concerned with obtaining new endophytic fungi isolates, testing their ability to inhibit the growth of Alternaria triticina the causal agent of leaf blight disease of wheat, and also their potential effects as growth promoting factors for wheat plants. In this study, some endophytic fungi isolates were isolated from different wild plant species in Saint Catherine protectorate and evaluated under laboratory conditions using in vitro dual culture technique against Alternaria triticina, Overall 72 endophytic fungal isolates have been isolated from inner tissues of wild plants and evaluated for their ability to control the growth of fungal pathogen, 17 isolates of them appeared effective to inhibit Alternaria triticina using dual culture technique. Most effective isolates were evaluated and screened in vitro for confirm potency where 10 isolates belonging different fungal species were high effective and confirmed namely Aspergillus niger, Chaetomium globosum, Cladosporium cladosporides, Curvularia tunata, Mucor plumbeus, Penicillium brevicompactum, Penicillium sclerotiorum, Penicillium sinaicum, Phoma glomerata and Ulocladium atrum from in vitro results 6 best isolates were selected for greenhouse experiments where showed that among of them 4 enophytic fungal isolates belongs C. globosum, Mucor plumbeus, Penicillium sinaicum and Ulocladium atrum  suppressed  Alternaria triticina. The endophytic fungi also enhanced the plant growth parameters of wheat plants like plant height, fresh and dry weight as compared to control.