This work was conducted in the experimental laboratory and apiary of the Plant Production Department  of the Faculty of Technology and Development ZagazigUniversity , Egypt. The main purpose of this work was to throw the light on the control of varroa mite,  Varroa destructor infected honey bee colonies using some  biological control agents. Six essential oils were used: Cinnamon oil, Clove oil, Jasmine oil, Eucalyptus  oil, Peppermint oil, in addition to Formic acid 65% , Oxalic acid and  Di stop. The obtained results reported that the increase percentages of dropped varroa numbers,  comparing with check , were the highest with the treatments of Cinnamon oil 78% ,while the least percentages was 61 % at Oxalic acid treatment. The decrease percentages of infected sealed broods per 100 cell ,  comparing with check , were the highest with Distop  38 % , while the least percentages was 25  % at Cinnamon oil. The highest increase percentages of infested bee workers , comparing with control treatment , was the highest with the treatment of Oxalic acid  41 % , while the least percentages was recorded with Di stop  giving  22%.   The highest increases in sealed brood area were noticed with Di stop  28 % , while the least increase percentages were recorded with jasmine . The highest increase percentages in honey bee production , in comparison with control hives, were registered with the treatments of Clove oils giving 171.4 %.