This study aims to control Charcoal root rot disease of tomato caused by Macrophomina phaseolina by using biocontrol agents and ecofriendly compounds. The results indicated that all tested plant water extracts reduced the linear growth and sporulation of Macrophomina Phaseolina both under laboratory and greenhouse conditions. Complete growth inhibition (100%) was observed in Macrophomina Phaseolina when cactus or clove extracts were applied at all tested concentrations (5, 10 and 15%). The best effective plant extract in reducing sclerotia population was nigella followed by clove. They resulted 40.0 and 34.2% reduction of sclerotia population respectively, compared to control. Also, the application of essential oils to soil previously infested with the pathogen at different tested concentrations (5, 10, and 15) significantly reduced disease incidence and sclerotia population of Macrophomina Phaseolina. The best results were obtained when mint oil was applied; followed by cloveoil. Trichoderma asperellum and Trichoderma koningii were the best tested Trichoderma spp. isolates in reducing the incidence and severity of disease Macrophomina phaseolina. All the above-mentioned treatments reduced the charcoal rot incidence and improved the growth of tomato plants; significantly under green house and artificial soil infestation conditions.