A method for simultaneous determination of penconazole content in some Emulsifiable Concentrate (EC) formulations has been described. Determination and quantification of penconazole were performed by gas chromatography equipped with a flame ionization detector (GC/FID) using an external standard of high and known purity. Validation parameters based on the Australian Pesticides and Veterinary Medicines Authority (APVMA) guidelines and ISO/IEC 17025 definition including method specificity, linearity, precision, accuracy, limit of detection (LOD) and limit of quantitation (LOQ) were evaluated. Under the optimum conditions, the linearity was found to be high with correlation coefficient values (R²> 0.999) for the target penconazole formulations. The results showed precise and accurate method. The RSD% was in the range of 1.40% for formulation of 200 g (a.i.)/L and 1.43 % for formulation 100 g (a.i.)/L respectively. Selectivity showed no interference from any other possible adjuvants or components. It can be concluded that the GC-FID described method is reliable, suitable and successfully applied to the estimation of the target penconazole determination. GC- MS and IR spectroscopy were used as a type of quality control to ascertain the presence of penconazole.