Tomato wilt disease caused by Fusarium oxysporum f.sp. lycopersici is one of the most destructive diseases, and  causes significant yield losses in Egypt. In the present study, the antifungal activities of some essential oils (clove, garlic and thyme) at various concentrations (10, 15 and 20 %) in addition to different  Trichoderma isolates(Trichoderma harzianum, Trichoderma hamatum and Trichoderma viride) and three different concentrations of urea (0.5 , 1 and 1.5%) was investigated against the pathogen (FOL).. The results revealed that the all tested essential oils reduced the growth and spore population of FOL significantly.  The highest pathogen reduction was recorded with clove oil followed by garlic oil either under laboratory or green-house conditions.  Contrary, application of urea fertilization at concentrations of 1% and 1.5% increased significantly both growth mass and sporulation capability of FOL comparing to the control. The highest percentage (94.2%) of FOL growth inhibition under in vitro condition was recorded with Trichoderma harzianum, followed by Trichoderma viride (90.8%). Similar results were recorded under greenhouse conditions.  Furthermore, the results showed that the all tested oils, Trichoderma isolates and urea fertilization improved the growth criteria of tomato plants comparing to the control plants. On the other hand, the histological investigation proved that Trichoderma harzianum and clove oil were the best treatments where there weren't histological changes in the external cortex, epidermal cells, endodermis and vascular vessel.