The present study was carried out to evaluate the relative insecticidal and biological activity of chitosan and its derivativesagainst the 2^nd larval instar of T. absoluta compared to the unprecedented insecticide chloroanatrilprole (Coragen) using standardized method of bioassay under laboratory conditions.  The larvacidal   activity for the different treatments against 2^nd instar larvae of   T. absoluta   were predestined. Also, effect of the tested agents on growth inhibition, antifeedant and life-cycle of the tested insect were determined. The results revealed that chitosan nanoparticles (NPs) was  the most effective treatment against 2^nd larval instar and  eggs deposited per gravid female of  T. absoluta. while, chitosan hydroxyapatite nanoparticles (NPs)  was the highest effective treatment on growth inhibition and antifeedant.  All tested agents disrupted life cycle of the tested insect under laboratory conditions. Therefore, this study suggests the possible using of chitosan and its derivativesas safe alternatives to conventional insecticide and compatible with integrated pest management practices.