The present study was conducted in the privet apiary established at Kafr Saad distances, Damietta Governorate, North Egypt, during summer season of 2019, (July and August). The experiments were carried out to determined the effect of genetic origin of the grafted larvae, (Apis mellifera carnica and Apis mellifera bukfast) and level of bar at which the queen cells hung within rearing frame, (top, medium and bottom levels) and the  position of these cells on the rearing bar (middle and peripheral positions) onsome output parameters concerned in commercial queens production. The genetic origin of larvae used in grafting process has a positive and significant effect on their acceptance percentage and the live weight of the produced queens when nursed by related starter and finisher building colonies. The percentages of acceptance, sealing and queen emergence as well as the weight of newly emerged virgin queens slightly influenced by the level and position at which the cells presented. In general, queen cells on the bottom bar and middle position were attained a little performance than the others in the commercial queen production during summer season of North Egypt.