To study the potential alternative hosts to Potato virus Y (PVY), thirteen different plant species, Solanum tuberosum cv. Spounta L., S. tuberosum cv. Cara L., Datura stramonium L., D. metel L., Nicotiana tabacum cv. White Burley L., Chenopodium amaranticolor (H.J.Coste & A.Reyn.) H.J.Coste & A.Reyn., Solanum lycopersicum L., S. melongena L., Capsicum annuum L., Phaseolus vulgaris L., Vicia faba L., Brassica oleracea L. and Lactuca sativa L., belonging to five botanical families were tested. Nine of the above tested plant species reacted to PVY isolate with different symptoms, while four of plant species D. stramonium, V. faba, B. oleracea and L. sativa did not react to PVY isolate. Mechanical transmission of PVY was confirmed from infected potato to healthy D. metel plants and back from PVY-inoculated D. metel to healthy potato plants with 100% success. Insect transmission was also confirmed from infected potato plants to healthy ones through aphid Myzus persicae with 80%. Virus particles of PVY were examined using transmission electron microscopy (TEM). Typical PVY particle size of 11 × 700 nm with flexuous filamentous shape was observed.