Laboratory and field experiments were carried out to evaluate the toxic effect and also the bioactivity of certain recently conventional pesticides (acarmite nano, acarmite common, Spirodiclofen nano, Spirodiclofen common, eucalypts extract nano eucalypts extract common, boletus satanas nano and boletus satanas common) against females adult and eggs for two spotted “spider mite" Tetranychus urticae, Koch. Moreover, to evaluate the sub lethal doses side effects of compounds tested on several bio-aspects of T. urticae, and evaluates of these toxicological actions of these compounds aginst testedpest T. urticae, on infested cottons under the field condition. Data obtained cleared that, the acarmite nanoparticles was the biggest toxic against females adult of T. urticae followed by common solution of acarmite.While nanoparticles of each eucalypts extract and boletus satanas give intermediate toxic to tested femoles of T.urticae .Common solution of eucalypts extractand boletus satanas were lowest action of toxic against T.urticae females.