The present investigation was achieved at the apiary of Sakha Agricultural Research Station, Kafr El-Sheikh Governorate during 2008 season to evaluate the efficiency of five materials, i.e., formic acid 60%, salcelate methyl 60%, Bayvarol strips, malathion powder 1% and Apitol for controlling varroa mite. All tested materials reduce mite population. Malathion powder 1% and formic acid 60% were the most effective material on varroa mite (93.75 & 89.57% reduction) on each of workers and drones after the fourth week of treatment during the first period from 1/2/2008 to 28/2/2008, respectively. Also, during the second period from 1/9/2008 until 28/9/2008, malathion powder 1% gave the highest reduction (93.15 & 94.00%) of varroa mite on each of workers and drones after the fourth week of treatment, respectively. On the other hand, the remaining materials, i.e., formic acid 60%, Apitol (2 gm), salcelate methyl 60% (2 cm³/colony) and Bayvarol strips gave a high reduction (88.19, 86.67, 84.08 & 82.0%) of varroa mite on workers after the four weeks of application during the first period, respectively. Also, during the second period formic acid 60%, salcelate methyl 60% (2 cm³/colony, Apitol (2 gm) and Bayvarol strips exhibited a high reduction (88.13, 88.11, 84.21 & 82.86%) of varroa mite on workers after the fourth week of application, respectively. At the same trend, formic acid 60%, salcelate methyl 60% (2 cm³/colony), Apitol (2 gm) and Bayvarol strips gave a high reduction (91.67, 91.11, 86.67 & 84.11%) of varroa mite on drones after four weeks of treatment during the second period, respectively. It could be concluded that bee keepers can use formic acid 60%, and salcelate methyl 60% (2 cm³/colony) safety during the above two tested periods for controlling varroa mite.