Matching of sixteen monogenic lines and twenty wheat (Triticum aestivum L.) varieties representing the Egyptian germplasm inoculated with thirty different stem rust isolates to postulate stem rust resistance gene (Sr). Genes were determined according to the infection types (IT) to different Puccinia graminis f. sp. tritici isolates for seedling resistance in wheat varieties. All of the tested varieties were probably present in Sr7b and Sr8a, with the exception of Giza 160 and Sohag-3, whereas, Sr9e gene were detected in Gemmeiza-7 but it was not detected in the rest of tested varieties. Thirteen genes were probably present in Gemmeiza-7 (the highest), however Sakha 61, Sohag-3 and Sakha 160 wee included the   least genes (Sr's). Sr29, Sr30, Sr36 followed by Sr7b and Sr8a were most commonly postulated and having the highest frequency, while Sr9e, Sr21, Sr26 SrT_t-1 appeared in lower frequencies within the used Egyptian wheat varieties.