A survey of plant parasitic nematodes associated with vineyards was undertaken in Sana'a and Sadah governorates, the two major grapevine-producing areas in Yemen. A total of 139 soil samples were collected and plant parasitic nematodes were extracted by a combination of sieving and Baerman-pan technique. Identification revealed presence of twenty four genera i.e., Antarctenchus, Aphelenchoides, Aphelenchus, Basiria, Criconemoides Ditylenchus, Dolichodorus, Gracilacus, Helicotylenchus, Hoplolaimus, Longidorus Meloidogyne, Paratylenchus, Pratylenchus Rotylenchulus, Rotylenchus Scutellonema, Telotylenchus, Tetylenchus, Trichodorus Tylenchorhynchus, Tylenchus, Xiphinema and Zygotylenchus. All nematode genera except Aphelenchoides on Asemi, Aphelenchus on Asemi and Raziki, Helicotylenchus on Black, Longidorus, Meloidogyne, Paratylenchus, Pratylenchus, Tylenchorhynchus and Xiphinema in Bani-Hushaisharereported for the first time on grape in Yemen. The most predominant plant nematode genera on grape cultivars in all locations were Tylenchus, Xiphinema, Helicotylenchus, Tylenchorhynchus, Rotylenchulus and Pratylenchus, while Paratylenchus and Meloidogyne had high frequency occurrencein Sana'a and Sadah governorates, respectively. In Sana'a governorate Paratylenchus and Xiphinema exhibited the highest population densities of 646 and405nematode/250gm soil withfrequencyoccurrence attaining54% and 67% respectively. In Sadah governorate Xiphinema and Meloidogyne had relatively high population densities of 502 and 429 nematode/250 gm soilswith 25% and 50%frequency occurrence, respectively