Biochemical and histological studies were executed on 4^th larval instar larvae of the Pink bollworm (PBW) and the spiny bollworm (SBW) treated as newly hatched larvae with LC₅₀ for two tested oils (Jojoba oil, and flaxseed oil). Results noticed that Jojoba oil and flaxseed oil decreased protein levels in Pectinophora gossypiella by 35.92 and 20.73% as compared with control, respectively. On the other hand, they caused increases in protein levels in Earias insulana, by 7.93% for Jojoba and by 4.50% for Flax. The total soluble lipids were estimated by -44.82and 45.86%, respectively for P. gossypiella, while they were -5.26 % in Jojoba and -11.48 in Flaxseed oil for E. insulana. Carbohydrate hydrolyzing enzymes activity was clearly increased by both treatments on E. insulana, while variations impacts on P. gossypiella were recorded (-34.19 % for Jojoba oil and 6.95% for Flaxseed oil). General increases in GOT and GPT transaminaes activity were recorded in P. gossypiella and E. insulana by due to the used oil treatments, but reduction percentages were found in alkaline phosphates as well as for Lactate dehydrogenase catalyzes (LDH) when the investigated pests treated with Jojoba and Flaxseed oils. Also, the histopathological disturbance was estimated in this study when neonate larvae fed on diet treated with LC₅₀ value of Jojoba oil and flaxseed oil. The cuticle and mid-gut tissues of the 4^th instar larvae showed vacuolization with hypertrophied lining mucosal epithelial, and the epithelial cells are blurred and Necrosis of nuclei.