114589

Alpha-Amylase Enzymes of Tribolium castaneum (Herbst) and Callosobruchus maculatus (F.) And Their Response to A-Amylase Inhibitor from Rice أنزبم الألفا أميليز فى حشرتى خنفساء الد

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

-

Abstract

Insect α-amylase has recently become a target of insect control strategies using plant-derived α-amylase inhibitors especially for the control of stored product insect pests. The first step to establish such strategy is to characterize the α-amylase enzymes in the target pest. In the current study, α-amylase was biochemically characterized in the larval and adult stages of Tribolium castaneum and Callosobruchus maculatus. T. castaneum was found to have higher α-amylase activity with lower temperature stability compared to that of C. maculatus. The optimum pH for α-amylase activity was 5-6. Zymogram pattern revealed the presence of two α-amylase isoforms with high molecule weight in T. castaneum. C. maculatus has two α-amylase isoforms in the adult stage and three isoforms in the larval stage. Rice α-amylase inhibitors was purified and found to have strong inhibitory effect against α-amylase of both insects and also negatively affected their life parameters.

DOI

10.21608/jppp.2020.114589

Keywords

Tribolium castaneum, Callosobruchus maculatus, α-amylase, α-amylase inhibitors

Authors

First Name

Ashraf

Last Name

Abd El-latif

MiddleName

Oukasha

Affiliation

Department of Plant protection, Faculty of Agriculture, Sohag University, Egypt

Email

ashrafoukasha@agr.sohag.edu.eg

City

-

Orcid

-

First Name

Nessma

Last Name

Mohieldeen

MiddleName

-

Affiliation

Department of Plant protection, Faculty of Agriculture, Sohag University, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Salman

MiddleName

Mahmoud

Affiliation

Department of Plant protection, Faculty of Agriculture, Sohag University, Egypt

Email

-

City

-

Orcid

-

Volume

11

Article Issue

8

Related Issue

16760

Issue Date

2020-08-01

Receive Date

2020-07-23

Publish Date

2020-08-01

Page Start

421

Page End

426

Print ISSN

2090-3677

Online ISSN

2090-3758

Link

https://jppp.journals.ekb.eg/article_114589.html

Detail API

https://jppp.journals.ekb.eg/service?article_code=114589

Order

8

Type

Original Article

Type Code

888

Publication Type

Journal

Publication Title

Journal of Plant Protection and Pathology

Publication Link

https://jppp.journals.ekb.eg/

MainTitle

Alpha-Amylase Enzymes of Tribolium castaneum (Herbst) and Callosobruchus maculatus (F.) And Their Response to A-Amylase Inhibitor from Rice أنزبم الألفا أميليز فى حشرتى خنفساء الدقيق الحمراء Tribolium castaneum (Herbst) وخنفساء اللوبيا Callosobruchus maculatus (F.) واستجابتهم لمثبط ألفا أميليز من الأرز

Details

Type

Article

Created At

22 Jan 2023