This study focused to produce new resistant rice genotypes to major rice diseases and its highly productivity. Rice accessions Giza 177, Sakha 105, Sakha 106 and Gz.7768 were used as male parents and TG-60-6 lines were used as female parent in addition to F_n lines obtained from four crosses. The best genotype has highly productivity and resistance to blast; TG-60-6/Sk.105-2, TG-60-6/Sk.106-3, TG-60-6/GZ.7768-2, TG-60-6/GZ.7768-5, TG-60-6/GZ.7768-6 and TG-60-6/GZ.7768-8. The results of yield and its compounds characters were influenced by environmental effect and the selection should be practiced successfully in late generation. Twenty four Pyricularia grisea isolates were identified as five main groups. Under artificial inoculation eleven genotypes were resistant to all rice blast races, but at field condition, eighteen genotypes were resistant at Sakha location and twenty two genotypes resistant at Gemmiza location.The biochemical changes of antioxidant enzymes and total protein were estimated after 24, 96 and 120 h of inoculation with blast race p23 (ID-15) at seedling stage. The maximum activity of perioxidase (POX), polyphenol oxidase enzyme (PPO) and total protein was recorded at 96 h after inoculation and then decreased. Under artificial inoculation with Bipolaris oryzae the causal fungus of brown spot disease, TG-60-6/G. 177-1 and TG-60-6/GZ.7768-2 rice genotypes were the lowest for disease severity. For artificial inoculation with Fusarium fujikuroi the casual fungus of bakanae, TG-60-6/GZ.7768-1and TG-60-6/G. 177-8 rice genotypes were the lowest seedling death percentages. TG-60-6/G. 177-8 rice genotype was proved the lowest disease incidence. While, the rice genotype TG-60-6/Sk.106-1 showed the lowest disease severity index.