The spores and supernatants of some fungal isolates; Paecilomyces violacea, Trichoderma harzianum andBeauveria bassiana;were evaluated to control the pink bollworm, Pectinophora gossypiella biologically at Plant Protection Research Institute, Sharkia branch, (ARC).All fungi were isolated from soil and dead pink bollworm larvae. B. bassiana was the most active isolate caused the greatest larval and pupal mortality, also the highest deformed adult, also, the lowest numbers of eggs and hatchability percentage. Protease and chitinase produced from B. bassiana. strain had high effects on percent of larval mortality, comparing with control. Identification ofBeauvaria sp. was assured by using 18s rRNA with accession number MT644472.1.