The entomopthogenic bacterium , Pseudomonas fluorescens, was used alone as soil treatment and foliar application or mixed with entomopthogenic fungus ,  Beauveria bassiana for controlling the two spotted spider mite ,Tetranychusurticae infesting Cucumberplants comparing with a standard synthetic acaricide ortus  . P. fluorescens great  effect as soil treatment and foliar spraying, recording reduction percentage of 75.21%. and no significant differences (df =6; P =<0.01) with the reduction caused by the acaricide used and this shows the importance of the role of biopesticides in reducing the population of T. urticae infesting CucumberPlants . There was significant effect on the lipase activities by all treatments, while the activity of chitinase shows that its activity increased at a high rate in all the treatments except ortus treatment compared with control. The acaricide, ortus had strong effect on the activity of acetylcholinesterase (AchE) where the activity of this enzyme decreased significantly (df =5; P =<0.01) to the extent of inhibition. While, the activity of this enzyme increased in the other treatments, where the activity reached nearly the control . The treatments which caused high reduction percentage gave high yield amount. There were nonsignificant differences in yield amount in treatment with (ortus), and (soil and foliar application of P. fluorescens), that indicates the important role of P. fluorescens in IPM against mite.