The study was conducted in four governorates namely Dakahlia, Behera, Gharbia and Alexandria, in order to study the effect of some natural substances on varroa mites, which affects honeybee colonies during the period from the first of August to the end of September, 2013. Results showed that the average percentage reduction in the incidence of varroa mite was 79.66%, 77.43%, 77.04% and 79.88% in the governorates of Dakahlia, Behera, Gharbia and Alexandria, respectively (in the brood + on adult bees) when using clove oil and menthol oil with concentration (12.5%،  25%). While the percentage of reduction of using eucalyptus oil in the same concentrations 65.6%, 73.8%, 70.9% and 66.73% for the previous governorates. From the previous findings it is advised to using one of these oils preferably clove oil for controlling varroa mite.