This work was conducted in apiary of Plant Protection Institute at Quanater, Qaluobia governorate. The study handled the effect of bee venom collection from honey bee colonies on royal jelly and honey production in spring season, 2013. Results showing that the honey bee colonies exposured to collecting bee venom gave less amounts of royal jelly and honey production than non-exposured honey bee colonies. There were significant differences between amounts of royal jelly and honey in exposure and non-exposured colonies to collect bee venom. All colonies were fed with sugar syrup and pollen supplements.