Stripe (yellow) rust caused by Puccinia striiformis f. sp. tritici (Pst) is one of the most important wheat diseases in Egypt and worldwide. Isolates of (Pst) collected during 2008/2009 and 2009/2010 were identified in 2009/2010 and 2010/2011 growing seasons, respectively. Seven physiological races were identified in the first season i.e., 0E0, 4E0, 16E2, 16E128, 16E130, 60E153 and 60E177; while in the second season, 13 physiological races were identified i.e., 0E0, 6E0, 2E0, 2E16, 4E0, 4E4, 6E5, 6E20, 18E16, 34E16, 34E20, 38E20 and 70E4. Race 0E0 was the most frequent one followed by 4E0 and 6E4. Results obtained showed that Yr1, Yr5, YrSU, and YrSP were the most resistant genes against yellow rust in both growing seasons, while Yr7, Yr6 and Yr6⁺ were the most susceptible genes. These results are substantially important for wheat breeding programs for disease resistance.