Three types of packaging materials i.e., high density polyethylene (143 g/m2-HDP), 0.1-mm thick Kraft paper bags and woven polyethylene (WP) were used in combination with different temperature storage (ambient temperature 25±3 ^oC, 10-12^oC and 5-6^oC in refrigerator) during 2 storage periods of 6 and 12 months. The interaction effect of different temperature and storage package types showed significant differences in associated seed borne fungi and chemical composition of the stored seed compared with the seeds in the control treatment stored at ambient temperature determined at zero time of storage. Associated fungi detected with sorghum seed by seed health test methods were Fusarium verticillioides, F. semitectum, F. oxysporium, Curvularia lunata, Aspergillus niger, A.  flavus, Penicillium sp, Alternaria  solani, A. alternata, Bipolaris sorghicola, Drechslera  oryzae and Epicoccum nigrum. Results indicated that the highest frequency of seed borne fungi associated with sorghum seed was recorded for samples stored in high density polyethylene (HDP) package under room temperature condition after 6 months followed by samples stored in Kraft paper package, the total seed borne count numbers were 129 and 109.33, respectively, while, storage in woven polyethylene (WP) package had the lowest total count (78.67) of seed borne fungi. As regard to sorghum seeds chemical component, data of storage experiments showed that storage at room temperature generally resulted in reduction of crude fiber, ash, oil, crude protein, germination percentage and oil quality while it showed increase of free fatty acid and acidic value especially in seed stored in HDP package. The weight of 100 kernels were also decreased and total infection count were increased under storage at room temperature specially with using HDP package type compared with control treatment (at zero time storage). In brief, the storage of sorghum seeds in woven polyethylene packages type under low temperature (5-6 ^oC) was very suitable and led to reduction of storage fungi and to keep seed chemical components and oil quality of the seeds.    Therefore, the need to maintain the temperature, relative humidity and type of storage package is important criteria of storage.