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87473

PRELIMINARY EVALUATION OF In vitro SECONDARY METABOLITES OF Sclerotium cepivorum, THE CAUSAL AGENT OF ONION WHITE ROT

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Last updated: 04 Jan 2025

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Abstract

 
Culture filtrates and organic solvent extracts of Sclerotium cepivorum liquid cultures were evaluated for their antifungal activities against the same fungus. Filtrates at 50% taken from 30 to 45-day-old cultures have reduced S. cepivorum growth by 51.9%. This treatment has also caused a reduction in the number of sclerotia by 98.6%. No sclerotial germination was observed on the media amended with the culture filtrates at all concentrations tested (10, 25 and 50%),.Among all organic solvent extracts, methanol extract was the strongest growth inhibitor against the fungal pathogen; it inhibited S. cepivorum growth by 82.2, 93.3, and 93.6% when used at concentrations of 400, 800, and 1200 ppm, respectively. At the same concentrations, ethyl acetate extract exhibited high inhibition to the fungal growth i.e., 85.56, 88.89, and 90.56%, respectively, while chloroform extract was the least effective in this regard causing  71.11% mycelial growth inhibition when used at concentrations of either 800 or 1200 ppm. All organic-solvent extracts completely suppressed the formation and germination of sclerotia at all concentrations. Further studies are needed to isolate different bioactive compounds from secondary metabolites of S. cepivorum.

DOI

10.21608/jppp.2013.87473

Keywords

antifungal activity, Sclerotium cepivorum, secondary metabolites, biological control

Authors

First Name

Mohamed

Last Name

El-Sheshtawi

MiddleName

-

Affiliation

Plant Pathology Department, Faculty of Agriculture, Mansoura University, El-Mansoura 35516, Egypt

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Orcid

-

First Name

Mona

Last Name

Zaghloul

MiddleName

G.

Affiliation

Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, El-Mansoura 35516, Egypt

Email

-

City

-

Orcid

-

First Name

E.

Last Name

Elsherbiny

MiddleName

A.

Affiliation

Plant Pathology Department, Faculty of Agriculture, Mansoura University, El-Mansoura 35516, Egypt

Email

-

City

-

Orcid

-

First Name

Amany

Last Name

Saad

MiddleName

S. M

Affiliation

Plant Pathology Department, Faculty of Agriculture, Mansoura University, El-Mansoura 35516, Egypt

Email

dramany83@gmail.com

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-

Orcid

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Volume

4

Article Issue

8

Related Issue

13131

Issue Date

2013-08-01

Receive Date

2020-05-07

Publish Date

2013-08-01

Page Start

721

Page End

730

Print ISSN

2090-3677

Online ISSN

2090-3758

Link

https://jppp.journals.ekb.eg/article_87473.html

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https://jppp.journals.ekb.eg/service?article_code=87473

Order

4

Type

Original Article

Type Code

888

Publication Type

Journal

Publication Title

Journal of Plant Protection and Pathology

Publication Link

https://jppp.journals.ekb.eg/

MainTitle

PRELIMINARY EVALUATION OF In vitro SECONDARY METABOLITES OF Sclerotium cepivorum, THE CAUSAL AGENT OF ONION WHITE ROT

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Article

Created At

22 Jan 2023