This study was carried out to investigate the toxicity and biochemical effects of two (biopesticides) biofly, abamectin and two organophosphates pesticides cadusafos and fenitrothion against root-knot nematode, Meloidogyne incognita egg filtrates and  second stage juveniles (J2) as well as on laboratory experiment, also the  inhibitory effect of the tested pesticides to acetylcholenesterase (AChE) and adenosine triphosphatase (ATPase) were determined. Results indicated that the tested pesticides have toxic action against Meloidogyne incognita second-stage juveniles (J2) and egg filtrates after 24 hrs  from application to 72 hrs and the toxicity increased with the time. Abamectin was the most toxic followed by ,fenitrothion cadusafos and  biofly was least in its toxicity to M. incognita  second stage juveniles (J2 )and egg filtrates  while the toxicity was depending on dose. The tested pesticides have inhibitory effect on AChE and ATPase activity and the inhibitory effect increased with AChE in case of abamectin and cadusafos while the  potency of the organophosphate pesticides to inhibit ATPase was  limited refers to its mode of action