Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most damaging foliardiseases of wheat world-wide. Nineteen powdery mildew differential monogenic lines (Pm) and ten durum wheat cultivars were evaluated for powdery mildew reaction at the seedling stage in a control-conditioned glasshouse and at adult stage under field conditions during 2018/2019 and 2019/2020 growing seasons in Gemmeiza Agriculture Research Station, ARC, Egypt. At seedling stage, Pm13, Pm24, Pm35, Pm36 and Pm37 were completely effective against 78 tested isolates of powdery mildew followed by Pm16, Pm32, Pm34, Pm29 and Pm43 according to their descending order. At adult stage, all the Pm genes were resistant except Pm8 and Pm9, which showed susceptibility to the disease. Although, the durum wheat cultivars were susceptible to powdery mildew isolates at seedling stage, they ranged from intermediate resistant to resistant at the adult stage. To confirm the presence of resistant genes in 10 Egyptian durum wheat cultivars, five specific molecular markers i.e. KSUG53, Xgwm337, Xcfd7, Bj261635, and Xgwm332 linked to Pm13, Pm24., Pm35., Pm36 and Pm37resistance genes were selected.  The linked markers used in this study assured the presence of Pm13, Pm36 and Pm37 in all tested durum cultivars. However, Pm35 was present in BeniSweif1, BeniSweif3, BeniSweif5 and BeniSweif6. Moreover, data showed that Pm24 was absent in all tested cultivars.