Lambda-cyhalothrin, Profenofos and Chlorpyrifos are a broad-spectrum pesticides extensively used to control pests for agricultural and household purposes. In the present study an attempt has been made to evaluate its toxicity profile, the cytotoxic, genotoxic and gene mutations effects in-vitro using structural chromosome aberration (SCA) and micronucleus (MN) test systems in erythrocytes assays in mice bone marrow cells. All doses of tested pesticides increased the number of structural chromosomal aberrations and the frequency of micronucleated erythrocytes compared with the control group. While, the results observed that tested pesticides caused a significant increase in the number of structural chromosome aberration and the frequency of micronucleus formation of the metaphase plates of the samples treated with the higher two concentration treatments of 1/10 and 1/40 LD₅₀ of all tested pesticides for 24 hour. In the case of micronucleus test the mice administered for 30, 60, and 90 days, the data revealed satellite associations, chromatid breaks and gaps indicating its effect on chromosomes compared with the control group. The acceptable daily intake (ADI) doses not induce any significant effect. It was also observed that, all tested pesticides induced significant increase in the frequency of chromosome aberration in the bone marrow cells which showed a significant dose-response correlation. Hence, its may be proposed that in-vitro assays like micronucleus and chromosomal aberrations test which indicate genetic damage could be used to study the toxic effect of organophosphorus and pyrethroid pesticides poisoning in humans.