Wheat stem rust is one of the major diseases on wheat in Egypt which appears annually in virulence of different frequencies. In this investigation, stem rust samples were collected from different locations of lower Egypt during two seasons (2008/09-2009/010). Three single pustules method of isolation was followed for each sample. Rust data were recorded as infection types, and virulence frequencies were determined against 35 Sr genes, in monogenic lines and some Egyptian genotypes. Virulence frequencies were very high against Sr21 +9g, Sr 7a, Sr 16, Sr 9e, Sr 6 and Sr 17, while the lowest frequencies were recorded against Sr 31, Sr 29 and Sr30. The physiologic races were identified according to their reaction on the 20 Sr^,s were recorded for each race. Thirteen and two physiologic races of Puccinia garminis were dantefiand. Race group TT- was the most frequent (16.16 %) , race group TS- (11.76%) ,race group KT- and TK- ( 5.88 %) followed by race group BB-, DH, PR, and SR (2.94% ). The least ones in the regard were races of group BC, BH