Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici, is one of the most devastating foliar diseases of wheat (Triticum aestivum) worldwide. Growing resistant cultivars is the best approach for control of the disease.  but only a few genes confer effective all-stage resistance against the current populations of the pathogen worldwide. It is urgent to identify new genes for diversifying sources of resistance genes and for pyramiding genes for different types of resistance in order to achieve high levels of durable resistance for sustainable control of stripe rust.  The objective of this study was to identify Inheritance of stripe rust resistance  in some Egyptian wheat cultivars.               Five crosses between Sakha 61 and each of Sakha 69, Giza 163, Gemmiza 5 Sids 7and 7 Sids 8 were performed. Seedlings of the parents , F1 and F2 were tested with P. striiformis f. sp. tritici races 172E155 under controlled greenhouse conditions. Crosses tested at seedling stage exhibited susceptible reaction against stripe rust. Under field condition plants segregation indicated that F₁ plants of the five crosses were resistant and exhibited low stripe rust severity ranged between 0 and 20 R. The result of F₂ plants reaction exhibited wide range of stripe rust severity ranged between 0 and 60 S but the direction was in the side resistance this confirmed the results of F₁. This study indicated that c.v. Sakha 61 also contains the stripe rust resistance gene at adult stage such as the tester proved to have.               The sergeaint analysis in amplified DNA polymorphism of tested wheat individuals using the primer (GACCGCTTGT) clearly showed that resistance gene was present in parent sakha61,f1and  F₂ of resistance and was absent  in f2 susceptible.