Laboratory studies were applied to investigate  the efficiency of bromadiolone 0.005%, vitamins (A, C and E) and bromadiolone 0.005% with vitamin E; on the both sexes of climb. rat, Rattus rattus alexandrines; the obtained results could be summarized in the following points;- The daily bait consumption of males and females of climbing rat, R. rattus had no any noticeable differences between the investigated vitamins A, C and E. ), Vitamin A and C had no mortality, meanwhile vitamin E at 0.50%, 0.75% and 1.0% caused 20%kill, 28%kill and 8.5%kill for male. Where   it was 16%kill, 20%kill and 16.0% kill for female of R. rattus, respectively. Vitamin E at 1.0 ratio killed each of male and female of R. rattus after 1.8 day and 4.4 day as average, respectively. The tested vitamins (A, C and E) in different mixing bait ratio 0.50%, 0.75% and 1.0%, caused reduction to the body weight of both sexes of climb. rat, Rattus rattus alexandrines especially Vitamin E, The daily bait consumption from bromadiolone 0.005% with 1.0% vitamin E of sub lethal doses, LD₅₀, for male and females R. rattus, were (8.5gm),. The mortality increased when the rats exposed to bromadilone plus Vitamin E than either of them singly. Also the highest mortality was recorded for bromadilone + Vitamin E 0.75% (gave 30%kill) Mostly, the shortest time to death under the combination condition was recorded by female.