The present study was performed in a private apiary at Ayash village, El-Mehalla EL-Kobra, GharbiaGov. during the dearth season from January up-to March, 2011. Three protein diets were used as pollen substitutes for honey bees, Apismellifera, feeding i.e. soybean flourmaizeflour and wheat flour (alternative protein diets), Also sucrose solution (sugar syrup;) (2 : 1 w/w) was added. All the cages were fed on the mentioned diets for 12 weeks.Results indicated that, significant differences were found between treatment groups in sealed brood rearing workers produced by colonies during the study period  when compared with the control treatment. Colonies feeding on soybean flour showed the increase  in the mean of brood rearing by 75.30 inch² followed by wheat flour (51.00 inch²) then maize flour (35.30 inch²) comparing with the control treatment (20.30 inch²). Soybean occupied the first rank in the consumption rate (48.46 gm /10 days) followed by 30.00 gm/10 days,in case of maize flour, while was(26.60 gm/10 days) in case of wheat flour. The consumption of sugar syrup increased with soybean diet (711.50 cm³)of sealed broad than the others. Also, The newly emergence workers fed on soybean cake showed the longest mean of age at (16 – 18 days)comparing with the other treatments.