Efficiency of profenofos, chlorpyrifos and alpha-cypermethrin against Pectinophora gossypiella and their associated predators in cotton field at Zagazig, Sharkia Governorate. Alpha-cypermethrin increased mean seasonal reduction percentages (92.92%), while, profenofos decrease it (78.38%). Alpha-cypermethrin caused the highest mean seasonal reduction percentages in the Coccineledae, Chrysoperla carnea and True spider predators (88.26, 73.67 and 85.70%, respectively).  The pupal mortality percentage increased while, pupal duration, adult emergency percentage, pupal weight, pupation percentage, deposited eggs and hatchability percentage decreased. The activity of trehalase, invertase and acetylcholine esterase enzymes decreased but of amylase and AST (Aspartate aminotransferase) increased. Disturbance recorded in ALT (Alanine aminotransferase) enzyme, total lipid and total soluble protein content relative to control.