Two field experiments were carried out at the experimental farm of the Faculty of Agriculture, Benha University, Qalubiya governorate during two seasons, 2015 and 2016. The experiments aimed   to study susceptibility of five soybean varieties (Giza 21, Giza 22, Giza 35, Giza111 and Crawford) under natural infestation with piercing sucking pests mainly; Bemisia tabaci (Genna).  Aphis sp., and Tetranychus urtica (Koch). Also to investigate relationship between these pests and some climatic factors and leaflets characteristic. The obtained results showed that, B. tabace started to appear on the 4^th week of June and reached the highest infestation was on 4^th July. The infestation by Aphis and T. urticae appeared earlier on 3^rd week of June and increased gradually until reached its peak on 3^rd July. Susceptibility of the tested soybean varieties to infestation with the pests varied significantly between each other. Crawford variety had the highest infestation (susceptible), except Giza. 21 with B.tabace in the 2^nd season. On the other hand, Giza 35 variety was more resistant with Aphis and T. urticae, the lowest infestation with B. tabace was found in Giza 22. Relationship among soybean cultivars, infestation level of temperature and humidity was significant. On the other hand, there was a negative significant relationship between the chlorophyll content and infestation with the pests.