Bee honeys are natural product from the honeybee colonies which has a great nutritional and medicinal effects. In this research, different types of Egyptian bee honey were collected from 3 governorates to study their antibacterial activities related to their contents from hydroxide and non-peroxide such as phenols. The results showed that each type of bee honey had characteristics which changed by geographical collected area. All the tested bee honey samples had an inhibitory activity against of Staphylococcus aureus growth. The majority of tested bee honey samples had no antimicrobial effect against Pseudomonas aeruginosa colonies except one sample (Sesame bee honey from Asyut governorate). Hydrogen peroxide is the primarily main factor for antimicrobial activity association. Undiluted tested bee honey was the highest inhibition for growth S. aureus bacterial, followed by high-to-low concentrations.