The effect of major herbicide used worldwide glyphosate was studied on some organs of mature male and female albino rats (Rattus norvegicus). The LC₅₀ was determined for glyphosate was 9.7% as crushed maize bait.Animals were fed on bait treated with (1\2 LC₅₀) for 15 days. The  organs were collected  two weeks after treatment and fixed in 10% formalin saline. The results revealed that the liver of male showed necrosis of some hepatocytes with infiltration of mononuclear inflammatory cells, while the liver of females showed necrosis of some hepatocytes and dilation of blood vessels surrounded by fibrin with activation of vankopfer cells. Hemorrhage, infiltration of mononuclear inflammatory cells and necrosis of cells lining renal tubes were observed in the kidney of male, while in  female ,granular degeneration changes of cells lining renal tubes with infiltration of mononuclear inflammatory cells and hyper cellularity of glomeruli with thrombosis of some blood vessels were  recorded . The lung of male showed  congestion of  blood vessels and thickening of its wall, hyper proliferation of cells lining bronchial, infiltration of mononuclear inflammatory cell and thickening of interstitial wall, whereas  hemorrhage, swelling of endothelial cells lining blood vessels, infiltration of mononuclear inflammatory cells and emphysema were observed in the lung of female.