Resistance development is a key part in pest management; Experimental trials included five conventional insecticides; Mospilan, Imidor (Neonicotinoid), Actellic (Organophosphate), Chess (Azomethine pyridines) and Aphox (Carbamate) to investigate their toxicity against both field and laboratory strains of Aphis craccivora Koch. The potency levels of the ongoing insecticides against the A. craccivora were 1.29, 1.43, 1.75, 1.97, 2.75 folds in laboratory strain more than that of field strain respectively. Thus, results showed that the field strain was more resistant for all compounds than that of laboratory strain. On the other hand, the results of the biochemical aspects of detoxification enzymes; Mixed Function Oxidase (MFO), Glutathione-S-transferases (GST), α and β-esterases demonstrated that, all tested insecticides induced impact on these detoxifying enzymes in both laboratory and field strain of A. craccivora.