Lupine is cultivated in Egypt for food, medical and industrial purposes. Root rot diseases caused by several soil-borne fungal pathogens are among the most destructive diseases attacking lupine plants. Greenhouse and field experiments were conducted to study the effect of hydrogen peroxide (H₂O₂) and acetylsalicylic acid (ASA) in addition to Rhizolex-T50 on lupine root rot disease, growth, certain physiological activities and productivity of lupine. Lupine seeds (cvs. Giza 1 and Giza 2) were examined. The data indicated that isolation of pathogenic fungi from both cultivars of diseased lupine was carried out in five locations of Dakahlia governorate. The high frequency isolated fungi presented in Temi El-Amdeed followed by Bani-Ebeed location. Fusarium solani and F. oxysporum proved to be the most dominate isolated followed by Rhizoctonia solani. In greenhouse, Giza 1 was high susceptible cultivar for infected with root rot pathogenic fungi. Sclerotium rolfsii followed by R. solani whereas F. solani was the most aggressive damping-off disease. In the field experiment, Giza 2 cultivar was the best in germination% and more tolerant of damping-off than Giza 1. The application of Rhizolex-T50 followed by H₂O₂ at low concentrate (0.50 mM) showed a highest percentage of germination within lowest percentage of damping-off. No significant differences between Rhizolex-T50 and H₂O₂ at 0.50 mM were detected. The high photosynthetic pigments and phenolic content were obtained from the application of ASA at moderate concentrate (15 mM) in both cultivars. Giza 2 gave the highest values in these parameters. Soaking in both tested materials increased significantly growth parameter examined, yield components and seed quality. The moderate concentration of ASA (15 mM) was the most effective followed by the low concentration of H₂O₂ (0.50 mM). Could be concluded that the application of H₂O₂ at 0.50 mM and ASA at 15 mM as seed soaking could be considered as fungicide alternatives for controlling lupine root rot disease as well as improve growth and productivity.